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Postdoctoral Scientist : Madrid, Spain

Open position at the “Single-molecule mechanobiochemistry” laboratory at CNIC, Madrid

We are looking for an experienced postdoctoral scientist to join the “Single-molecule mechanobiochemistry“ laboratory led by Jorge Alegre-Cebollada at the National Institute for Cardiovascular Research (CNIC) in Madrid, Spain. The position will be available from July 2014 and will extend for 5 years. Our laboratory studies the role of elastic proteins in the development of cardiac disease, by combining the latest single-molecule manipulation techniques by Atomic Force Microscopy, and protein biochemistry techniques such as mass-spectrometry. The successful candidate will lead the identification and mechanical characterization of posttranslational modifications that control the elasticity of heart muscle. He/she will also participate in supervising students and technical staff, and will be involved in preparation of grant proposals. The CNIC offers a competitive salary and excellent benefits, together with the possibility to attend career development activities.

Only candidates holding a PhD in Biochemistry or a similar discipline, with at least 4 years of postdoctoral experience, will be considered. Additional requirements are summarized below:

•extensive experience in recombinant protein production, purification and biochemical characterization.
•extensive experience in single-molecule manipulation techniques (force-spectroscopy).
•strong track record of publications in international journals, including several publications as 1st author.
•experience in high-resolution microscopy techniques will be positively considered.

Applications should be sent to jalegre@cnic.es and will include a cover letter, a CV and the e-mail addresses of 3 potential references.

Deadline for proposal presentation: May 15th, 2014

The CNIC is a center that advocates equal job opportunities for men and women, foreign and national, and promote nondiscrimination by reason of age, race or ethnic origin, religion or belief, sexual orientation, language, disability, political orientation or social or economic conditions. The CNIC ensures absolute confidentiality in the selection process and the utmost rigor in the application of the principles of equality, merit and ability in public employment and current rules on data protection.

Selected publications:

1. Alegre-Cebollada, J., et al. S-glutathionylation of cryptic cysteines enhances titin elasticity by blocking protein folding. Cell 156, 1235-1246 (2014)

2. Kosuri, P., Alegre-Cebollada, J. et al. Protein folding drives disulfide formation. Cell 151, 794-806 (2012).

3. Alegre-Cebollada, J., et al. Direct observation of disulfide isomerization in a single protein. Nat Chem 3, 882-887 (2011)….

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