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Postdoctoral Training Fellow : London, United Kingdom

Postdoctoral Training Fellow

Human Embryology and Stem Cell Biology

Niakan Lab

This is a full-time, fixed term position for 4 years on Crick Terms & Conditions of Employment.

Salary between £33,500 – £36,600 per annum, subject to skills and experience.

The Francis Crick Institute (the Crick) is a partnership between the Medical Research Council (MRC), Cancer Research UK (CRUK), the Wellcome Trust, University College London (UCL), Imperial College London and King’s College London. It is a registered charity whose purpose is to conduct biomedical research into all aspects of human health and disease.

The institute will be a world-leading centre of biomedical research and innovation. It will promote connections between researchers and disciplines and between academic institutions, healthcare organisations and businesses. Dedicated to research excellence, the institute will have the scale, vision and expertise to tackle the most challenging scientific questions underpinning health and disease. It will be world-class with a strong national role — training scientists and developing ideas for public good. On 1 April 2015, staff from the London Research Institute (CRUK) and National Institute for Medical Research (MRC) transferred to the Crick to form a fully functional research institute on four sites. In 2016, the Crick will move to a single new, purpose-built research centre in St. Pancras, which will house some 1,500 staff.

We seek a talented and motivated postdoc in the area of human embryology and stem cell biology to join Dr. Kathy Niakan’s laboratory.

Organisation

Dr Niakan’s laboratory focuses on understanding the mechanisms of lineage specification in human embryos and the derivation of novel human stem cells. Details of research projects currently being undertaken can be seen at: http://www.crick.ac.uk/kathy-niakan

Research techniques used in the laboratory include: molecular biology, advanced microscopy and image quantification, human and mouse preimplantation embryo culture and micromanipulation, genome-wide techniques including single-cell RNA-sequencing, human embryonic and induced pluripotent stem cell derivation.

Objectives

The research aims are to characterise gene expression patterns during human preimplantation development and to develop conditions for the establishment of novel human stem cells.

The post holder should embody and demonstrate our core Crick values: Bold, Imaginative, Open, Dynamic and Collegial, in addition to the following:

• Genomic profiling of early human embryos and microdissected cells 
• Stem cell derivation from embryos 
• Reprogramming using induced pluripotent stem cell approaches
• Genome editing using CRISPR-Cas9, lentivirus transduction
• Ensuring the design and implementation of the project
• Liaising with collaborators within the Crick, the UK and abroad
• Writing and contributing to the preparation of scientific manuscripts, reports, presentations and records of experimental plans and results
• Working closely with the Group Leader and other team members to report on the results via publications
• Supervising and providing technical advice to more junior members of the team when appropriate

Person Specification

Essential:

• PhD in the areas of Developmental Biology, Stem Cells, Molecular Biology or similar (or in the final stages of PhD submission)
• Good knowledge and experience in molecular biology and microscopy
• Technical expertise in embryo and/or cell culture
• Proven track record of research (i.e. publication record)
• Strong written and oral communication skills
• Ability to prioritise and manage his/her own time
• Ability to work independently and also capable of interacting within a group
• Ability to design experiments, report on research progress and outcomes openly and review methodologies in response to feedback

Desirable:

• Experience in lentivirus production and transduction
• Experience in genome editing using CRISPR-Cas9 technology
• Experience in preimplantation mouse or human embryo culture
• Experience in human and mouse pluripotent stem cell culture
• Experience in preparing samples for advanced sequencing

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