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瑞典于默奥大学2021年招聘博士后职位（分枝杆菌核糖体生物学研究）

Postdoctoral Position To Study Mycobacterial Ribosome Biology (Cryo-EM)

Umea University

Project description and work tasks

The project will involve the characterization of the translation apparatus from Mycobacteria using in-vivo assays, RNA-protein biochemistry, and cryo- electron microscopy. The goal is to identify and study ribosome-associated factors in an unbiased way, provide structural information and uncover unique species-specific mechanisms that could later serve as novel targets for antibiotics.

Qualifications

The candidate must have a Ph.D. in Biochemistry, Biophysics, Microbiology, or similar. The Ph.D. degree should not be awarded more than three years ago at the time of application. Exceptions include illness, parental leave, clinical practice, appointments of trust in trade unions or similar circumstances, which must be stated in the application. Hands-on expertise in molecular biology, biochemistry, and microbiology in general, and ideally in Mycobacterial biology, is required. The ideal candidate has a background in Mycobacteria culturing (e.g., large-scale culturing of M. smegmatis or similar organisms for protein overexpression) and genomic engineering (e.g., has successfully used modern techniques for genome manipulation to obtain knockouts or knock-ins). Also, experience with bacterial expression and purification of recombinant proteins (ideally in M. smegmatis) and RNA/protein biochemistry is required. A background in structural biology is needed (i.e., preferably cryo-EM or alternatively X-ray crystallography). Excellent communication skills in spoken and written English and a high level of interpersonal skills are required, and the ability to work as part of a team as well as independently is expected.

Desirable qualifications

For the advertised project, knowledge of cryo-electron microscopy sample preparation, data collection, and processing will be a significant advantage.

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